In the cholesterol oxidase-peroxidase method, cholesterol oxidase reacts with which form?

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Enhance your knowledge with the Ciulla Clinical Chemistry Test. Study with interactive quizzes, featuring flashcards and multiple-choice questions. Each question offers hints and explanations for better understanding. Prepare effectively and excel in your exam!

Multiple Choice

In the cholesterol oxidase-peroxidase method, cholesterol oxidase reacts with which form?

Explanation:
Cholesterol oxidase specifically acts on free cholesterol. In the cholesterol oxidase–peroxidase assay, any cholesteryl esters in the sample must first be hydrolyzed by cholesterol esterase to free cholesterol; only then is the cholesterol oxidase able to oxidize it and generate hydrogen peroxide. That hydrogen peroxide is then used by peroxidase to produce a colored product proportional to the cholesterol concentration. So the form that cholesterol oxidase reacts with is free cholesterol, not cholesteryl esters.

Cholesterol oxidase specifically acts on free cholesterol. In the cholesterol oxidase–peroxidase assay, any cholesteryl esters in the sample must first be hydrolyzed by cholesterol esterase to free cholesterol; only then is the cholesterol oxidase able to oxidize it and generate hydrogen peroxide. That hydrogen peroxide is then used by peroxidase to produce a colored product proportional to the cholesterol concentration. So the form that cholesterol oxidase reacts with is free cholesterol, not cholesteryl esters.

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