In serum protein electrophoresis, when a buffer solution of pH 8.6 is used, which of the following characterizes the proteins?

Unlock all questions

This demo includes only 20 questions. Upgrade to access hundreds of questions, flashcards, exam simulations, and disable ads.

Full question bankExam simulationsFlashcards
From $24.99Unlock all

Enhance your knowledge with the Ciulla Clinical Chemistry Test. Study with interactive quizzes, featuring flashcards and multiple-choice questions. Each question offers hints and explanations for better understanding. Prepare effectively and excel in your exam!

Multiple Choice

In serum protein electrophoresis, when a buffer solution of pH 8.6 is used, which of the following characterizes the proteins?

Explanation:
The key idea is how a protein’s charge depends on pH relative to its isoelectric point. At pH 8.6, most serum proteins are above their pI, so they lose protons and carry a net negative charge. That negative charge is what electrophoresis separates by, and it explains why the proteins are characterized as having a net negative charge at this pH. In this setting, moving toward the cathode would require a net positive charge, which isn’t the case here, while neutral charge would occur near the protein’s isoelectric point.

The key idea is how a protein’s charge depends on pH relative to its isoelectric point. At pH 8.6, most serum proteins are above their pI, so they lose protons and carry a net negative charge. That negative charge is what electrophoresis separates by, and it explains why the proteins are characterized as having a net negative charge at this pH. In this setting, moving toward the cathode would require a net positive charge, which isn’t the case here, while neutral charge would occur near the protein’s isoelectric point.

More Multiple Choice Questions
Subscribe

Get the latest from Examzify

You can unsubscribe at any time. Read our privacy policy