Capillary electrophoresis relies on which phenomenon to move liquid through the capillary?

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Enhance your knowledge with the Ciulla Clinical Chemistry Test. Study with interactive quizzes, featuring flashcards and multiple-choice questions. Each question offers hints and explanations for better understanding. Prepare effectively and excel in your exam!

Multiple Choice

Capillary electrophoresis relies on which phenomenon to move liquid through the capillary?

Explanation:
Capillary electrophoresis relies on electro-osmotic flow to move liquid through the capillary. The capillary wall is typically negatively charged, creating an electric double layer. When voltage is applied, ions in that layer migrate toward the opposite electrode and, in doing so, drag the surrounding liquid along. This bulk flow moves the entire solution toward the detector and enables separation of analytes. Brownian motion and diffusion are random or gradient-driven processes that don’t produce the directed, field-driven flow seen here, and convection requires external stirring or heating. So the movement of liquid in this system is due to electro-osmotic flow.

Capillary electrophoresis relies on electro-osmotic flow to move liquid through the capillary. The capillary wall is typically negatively charged, creating an electric double layer. When voltage is applied, ions in that layer migrate toward the opposite electrode and, in doing so, drag the surrounding liquid along. This bulk flow moves the entire solution toward the detector and enables separation of analytes. Brownian motion and diffusion are random or gradient-driven processes that don’t produce the directed, field-driven flow seen here, and convection requires external stirring or heating. So the movement of liquid in this system is due to electro-osmotic flow.

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