Bisalbuminemia is detected by which laboratory technique?

• A. Serum protein electrophoresis
• B. Urine protein electrophoresis
• C. Immunoelectrophoresis
• D. Gel filtration chromatography

Answer: (A)

Bisalbuminemia shows two different albumin species in the serum, which split the albumin band into two separate bands when proteins are separated by charge in a gel or capillary. Serum protein electrophoresis specifically visualizes these bands in the albumin region, making it the method that reveals the double albumin pattern directly. The other options don’t focus on the serum albumin pattern: urine electrophoresis looks at proteins in urine, immunoelectrophoresis detects antigen–antibody patterns, and gel filtration chromatography separates proteins by size but is not the standard test used to identify this serum albumin variant. So the technique that best detects bisalbuminemia is serum protein electrophoresis.